661 8 1492 2 8371 document 1492 Development of a qPCR assay and tremabiome deep am.zip application/zip f046bdfeca113f34232a28440dbcad9b MD5 2434545947 2026-02-23 09:33:19 https://data.lancashire.ac.uk/id/eprint/661/1/Development%20of%20a%20qPCR%20assay%20and%20tremabiome%20deep%20am.zip 661 1 1 application/zip application/zip Full Dataset en public cc_by_4
Development of a qPCR assay and tremabiome deep am.zip
full_archive TRUE archive 8084 disk0/00/00/06/61 2026-02-24 16:07:40 2026-02-24 16:07:40 2026-02-24 16:07:40 data_collection show Abbas Muhammad Kozel Kezia Daramola Olukayode ODaramola1@uclan.ac.uk 0000-0002-3634-4556 Selemetas Nick Ali Qasim Ashraf Shoaib Ibrahim Isah Desa-Cruz Inaki Fingerhood Sal Robinson Mark Morgan Eric R Chaudhry Umer Betson Martha Development of a qPCR assay and tremabiome deep amplicon sequencing method for differentiation of fluke species in livestock pub D990 2023sch_VM Development of a qPCR assay and a tremabiome deep amplicon sequencing for fluke species differentiation in the UK livestock 2026-01-09 published Mendeley Data https://doi.org/10.17632/zyvwc6ppy8.2 collection FBS2022-Betson Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 Rapid Diagnostics for Neglected Parasites. Sir Halley Stewart Trust http://dx.doi.org/10.13039/501100000862 2023Inst_BEST rdmsupport@lancashire.ac.uk Veterinary Lecturer in One Health Daramola Olukayode ODaramola1@uclan.ac.uk Development of a qPCR assay for Fasciola spp. identification and deep amplicon sequencing method for differentiation of fluke species in UK livestock pub https://doi.org/10.1371/journal.pntd.0014006 https://knowledge.lancashire.ac.uk/id/eprint/58426/ Faecal sedimentation, DNA extraction, PCR using ITS-2 rDNA marker targeting coding regions of 5.8S and 28S rDNA, Next generation sequencing library prep, Next generation sequencing via MiSeq, Illumina MiSeq data analysis in Mothur/1.41.0-Python-2.7.15, R script using libraries phylotools, microseq, tidyverse, seqRFLP, readxl, writexl, ggh4x, Biostrings package, and NCBI BLASTn loop command using “blastn: 2.16.0+” in the HPC cluster system