Faecal sedimentation, DNA extraction, PCR using ITS-2 rDNA marker targeting coding regions of 5.8S and 28S rDNA, Next generation sequencing library prep, Next generation sequencing via MiSeq, Illumina MiSeq data analysis in Mothur/1.41.0-Python-2.7.15, R script using libraries phylotools, microseq, tidyverse, seqRFLP, readxl, writexl, ggh4x, Biostrings package, and NCBI BLASTn loop command using “blastn: 2.16.0+” in the HPC cluster system