459 7 archive 7024 disk0/00/00/04/59 2024-07-09 11:19:55 2024-07-09 11:24:31 2024-07-09 11:19:55 data_collection show Gent L Chiappetta ME Hesketh S Palmowski P Porter A Bonicelli Andrea ABonicelli@uclan.ac.uk Schwalbe EC Procopio Noemi NProcopio@uclan.ac.uk 2023sub_L The application of human bone in forensic proteomics is an expanding novel purpose in the aim of successfully quantifying biological estimations used in medico-legal investigations with greater accuracy. In this project, different extraction protocols were tested on n=30 human bone samples, including S-Trap technology with two different lysis buffer, and ZipTips. Additionally, a comparison was made between data-dependent acquisition (DDA) and data-independent acquisition (DIA) mode. Results showed less missing data using S-Traps instead of the more routine reverse-phase media tips (ZipTip). The type of lysis buffer when using S-Traps does not impact largely the analysis conducted in forensic proteomic workflows. Lastly, it was found that when open-source software is used for data processing for both DDA and DIA modes, DIA has the upper advantage in terms of acquiring a larger number of proteins due to its greater sensitivity to those with a lower abundance. dataset rdmsupport@uclan.ac.uk ds_ext 10.25345/C5QN5ZP1H DOI https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000093803 pub 10.1021/acs.jproteome.4c00151 DOI https://doi.org/10.1021/acs.jproteome.4c00151