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Abbas, Muhammad and Kobel, Kezia and Selemetas, Nick and Daramola, Olukayode and Morgan, Eric R and Chaudhry, Umer and Betson, Martha (2026) Limited genetic structure and high gene flow in Fasciola hepatica populations infecting ruminants in different geographic areas in the UK. [DataSet]
Description
The liver fluke, Fasciola hepatica, is a major parasitic threat to ruminant health and productivity worldwide, with important implications for food security, animal welfare, and zoonotic risk. This study developed and validated a multiplex deep amplicon sequencing assay targeting the mitochondrial NADH dehydrogenase 1 (mt-ND1) and cytochrome c oxidase subunit 1 (mt-COX1) loci for high-throughput genotyping of F. hepatica. DNA was extracted from eggs sedimented from sheep and cattle faeces (n = 78) received from farms and from adult worm pools (n = 12) isolated at abattoirs from diverse regions across the UK. Following high-throughput sequencing, bioinformatics analysis was performed to demultiplex Illumina sequence reads and extract amplicon sequence variants (ASVs). A total of 11 ASVs were identified at each locus (mt-ND1: 264–279 bp; mt-COX1: 312–319 bp), with two or three predominant ASVs per locus, along with rare variants. Network and PCA analyses revealed two distinct clusters at the mt-ND1 locus: one primarily associated with sheep and another shared between sheep and cattle. In contrast, mt-COX1 sequence reads formed a single dominant cluster. Population analyses revealed extensive ASV sharing across regions, indicating high gene flow, likely facilitated by livestock movement and parasite adaptation.
| Research / Data Type: | Collection - various types |
|---|---|
| Depositing User: | Christopher Waddington |
| Date Deposited: | 21 Aug 2026 13:40 |
| Revision: | 7 |
| URI: | https://data.lancashire.ac.uk/id/eprint/711 |
Related Resources
Limited genetic ... areas in the UK |
