Abbas, Muhammad and Kozel, Kezia and Daramola, Olukayode and Selemetas, Nick and Ali, Qasim and Ashraf, Shoaib and Ibrahim, Isah and Desa-Cruz, Inaki and Fingerhood, Sal et al (2026) Development of a qPCR assay and tremabiome deep amplicon sequencing method for differentiation of fluke species in livestock. [DataSet]

Description

Development of a qPCR assay and a tremabiome deep amplicon sequencing for fluke species differentiation in the UK livestock

Research / Data Type:	Collection - various types
Research Institutes:	BEST Health - (Biomedical Evidence baSed Trans-disciplinary)
Depositing User:	Christopher Waddington
Date Deposited:	24 Feb 2026 16:07
Revision:	8
URI:	https://data.lancashire.ac.uk/id/eprint/661
Additional details Additional details Creators: Creators Email ORCID Abbas, Muhammad UNSPECIFIED UNSPECIFIED Kozel, Kezia UNSPECIFIED UNSPECIFIED Daramola, Olukayode ODaramola1@uclan.ac.uk 0000-0002-3634-4556 Selemetas, Nick UNSPECIFIED UNSPECIFIED Ali, Qasim UNSPECIFIED UNSPECIFIED Ashraf, Shoaib UNSPECIFIED UNSPECIFIED Ibrahim, Isah UNSPECIFIED UNSPECIFIED Desa-Cruz, Inaki UNSPECIFIED UNSPECIFIED Fingerhood, Sal UNSPECIFIED UNSPECIFIED Robinson, Mark UNSPECIFIED UNSPECIFIED Morgan, Eric R UNSPECIFIED UNSPECIFIED Chaudhry, Umer UNSPECIFIED UNSPECIFIED Betson, Martha UNSPECIFIED UNSPECIFIED Schools: School of Veterinary Medicine* Date: 9 January 2026 Date type: Publication Data collection method: Faecal sedimentation, DNA extraction, PCR using ITS-2 rDNA marker targeting coding regions of 5.8S and 28S rDNA, Next generation sequencing library prep, Next generation sequencing via MiSeq, Illumina MiSeq data analysis in Mothur/1.41.0-Python-2.7.15, R script using libraries phylotools, microseq, tidyverse, seqRFLP, readxl, writexl, ggh4x, Biostrings package, and NCBI BLASTn loop command using “blastn: 2.16.0+” in the HPC cluster system Projects: Grant Number Funder Name Funder ID FBS2022-Betson Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 Rapid Diagnostics for Neglected Parasites. Sir Halley Stewart Trust http://dx.doi.org/10.13039/501100000862 Status: Published Publisher: Mendeley Data Contact email address: rdmsupport@lancashire.ac.uk Last Modified: 24 Feb 2026 16:07

Available Files

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Development%20o ... 20deep%20am.zip Development%20o ... 20deep%20am.zip Development%20o ... 20deep%20am.zip Visible to: Anyone Document Type : Full Archive File Description: Full Dataset Metadata Revision: 2 Mime-Type: application/zip Language: English License: Creative Commons: Attribution 4.0 File size: 2GB

Related Resources

Development of ... in UK livestock Development of ... in UK livestock Development of ... in UK livestock Resource Title: Development of a qPCR assay for Fasciola spp. identification and deep amplicon sequencing method for differentiation of fluke species in UK livestock Resource Type: Publication URL: https://doi.org/10.1371/journal.pntd.0014006 CLoK URL: https://knowledge.lancashire.ac.uk/id/eprint/58426/